Aim:- Biological synthesis of silver nano particles.

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Aim:- Biological synthesis of silver nanoparticles.

Requirements:-

  • Chemicals:- (1) Leaves for preparation of plant extract (either Marwa or Neem).

(2) Silver nitrate ( AgNO3)

(3) Glycerine.

(4) Double distilled water.

  • Equipment:- Beaker, Conical flask, Glass rod, Measuring cylinder, Test tube, Pipettes etc.
  1. Procedure:-  Marwa (Origanum majorana) and Neem (Azadirachta indica) leaves weighing about 20gm are thoroughly washed and dried and add 100ml sterile distilled water.
  2. Boil in a water bath for one hour at 600C.
  3. Filter through Whatman filter paper whose pore size is 0.45μm.
  4. Followed by further filtration through 0.22 μm sized pore size.
  5. 1mM silver nitrate is prepared in 100ml of deionised water.
  6. 10ml of plant extract was added into 90ml of the aqueous solution of 1mm silver nitrate.
  7. Mixture solution stirred for one hour.
  8. Silver nanoparticles are formed.
  9. Centrifuged the solution at 5000 rpm for 20 min.
  10. Lyophilize the nanoparticles solution.
  11. Store powdered silver nanoparticles at 40C to 80C.

UV-V’s Analysis- The optical property of silver nanoparticles is determined by UV-V’s spectrophotometer. The spectra are taken in different time intervals up to 24 hours between 350 nm to 500 nm.

 

Biological synthesis of silver nanoparticles has been done.

Result:- Biological synthesis of silver nanoparticles have been done.

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