Synthesis of Polymeric Nanoparticles- Objective, Equipment, Procedure, Result

Aim:- The synthesis of polymeric nanoparticles. The Synthesis of Polymeric Nanoparticles- Aim, Objective, Principal, Equipment, Procedure, Media Used, Result Interpretation, Limitations and Examples|

Requirement:-

Chemicals:- Berberine drug, Methanol, Sodium alginate, Tween 80, Distilled water.

Equipment:- Pipettes, Stirrer, Glass flask, measuring cylinder etc.

Procedure:-

For drug-loaded:-

1. Take 0.02gm of Berberine drug and added with 10ml of methanol (solⁿ– 1).
2. Stirrer the solution of step first for 5 to 10 minutes.
3. Now, take0.01gm of sodium alginate and added with 20ml distilled water. Stirrer the solution for 20-25 minutes at the speed of 300-500 rpm (solⁿ– 2).
4. Take 1.5ml of tween 80 and added to 100ml distilled water. Stirrer the solution for 15 minutes at 500-700rpm. Take 50 ml solution from this. (solⁿ– 3)
5. Now mix solution-1 into solution-2 drop wisely and now mix this prepared solution in solution-3 drop wisely.

Without drug:-

1. Take 10ml of methanol. (solⁿ-1)
2. Prepare solⁿ-2 same as in case of drug-loaded.
3. Take 50ml of Tween 80(solⁿ-3).
4. Now mix solⁿ– 1 into solⁿ– 2 dropwise and this prepared solution into solⁿ-3 drop wisely.

• Now take the PSA analysis and UV-V’s spectrophotometric analysis of both drug-loaded and without drug samples.

Result:-

Polymeric nanoparticles have been synthesized.